Sodium phosphate dibasic
SIGMA/71636 - BioUltra, Molecular Biology, ≥99.5% (T)
Synonym: sec-Sodium phosphate; Disodium hydrogen phosphate; Disodium phosphate; Sodium hydrogenphosphate
CAS Number: 7558-79-4
Empirical Formula (Hill Notation): Na2HPO4
Molecular Weight: 141.96
EC Number: 231-448-7
MDL Number: MFCD00003496
Linear Formula: Na2HPO4
Product Type: Chemical
| λ | 0.5 M in H2O |
| anion traces | chloride (Cl-): ≤10 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.5% (T) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.2 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤10 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | coarse powder |
| fine crystals | |
| powder | |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| ≤0.001% total nitrogen (N) | |
| InChI | 1S/2Na.H3O4P/c;;1-5(2,3)4 |
| InChI key | BNIILDVGGAEEIG-UHFFFAOYSA |
| loss | ≤0.1% loss on drying, 110 °C |
| pH | 8.8-9.4 (25 °C, 0.5 M in H2O) |
| pKa (25 °C) | (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid) |
| product line | BioUltra |
| Quality Level | 200 ![]() |
| SMILES string | [Na+].[Na+].[H]OP([O-])([ |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: ≤0.025 |
| λ: 280 nm Amax: ≤0.020 |
| Application: | Sodium phosphate dibasic has been used: • as a component in phosphate buffer • in the preparation of MgCl2 hybridization buffer • as a component in SSPE solution for the preparation of small RNA hybridisation buffer |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (T) |
| UNSPSC | 12161700 |

