Sodium dodecyl sulfate solution
SIGMA/71736 - BioUltra, for molecular biology, 10% in H2O
Synonym: Lauryl sulfate sodium salt; SDS; Sodium lauryl sulfate solution
CAS Number: 151-21-3
Empirical Formula (Hill Notation): C12H25NaO4S
Molecular Weight: 288.38
MDL Number: MFCD00036175
Linear Formula: CH3(CH2)11OSO3Na
Product Type: Chemical
λ | neat |
anion traces | chloride (Cl-): ≤50 mg/kg |
phosphate (PO43-): ≤1 mg/kg | |
cation traces | Al: ≤1 mg/kg |
As: ≤0.1 mg/kg | |
Ba: ≤1 mg/kg | |
Bi: ≤1 mg/kg | |
Ca: ≤5 mg/kg | |
Cd: ≤1 mg/kg | |
Co: ≤1 mg/kg | |
Cr: ≤1 mg/kg | |
Cu: ≤1 mg/kg | |
Fe: ≤1 mg/kg | |
K: ≤20 mg/kg | |
Li: ≤1 mg/kg | |
Mg: ≤1 mg/kg | |
Mn: ≤1 mg/kg | |
Mo: ≤1 mg/kg | |
Ni: ≤1 mg/kg | |
Pb: ≤1 mg/kg | |
Sr: ≤1 mg/kg | |
Zn: ≤1 mg/kg | |
concentration | 10% in H2O |
density | 1.01 g/mL at 20 °C |
description | anionic |
form | liquid |
grade | for molecular biology |
impurities | DNases, none detected |
insoluble matter, passes filter test | |
phosphatases, none detected | |
proteases, none detected | |
RNases, none detected | |
InChI | 1S/C12H26O4S.Na/c1-2-3-4- |
InChI key | DBMJMQXJHONAFJ-UHFFFAOYSA |
mol wt | 288.38 g/mol |
pH | 5.0-6.5 |
product line | BioUltra |
Quality Level | 200 |
SMILES string | [Na+].CCCCCCCCCCCCOS([O-] |
technique(s) | HPLC: suitable |
protein quantification: suitable | |
UV absorption | λ: 260 nm Amax: 0.3 |
λ: 280 nm Amax: 0.2 |
Application: | Sodium dodecyl sulfate solution has been used: • in the preparation of 2x extraction buffer • as a component in elution buffer for immunoprecipitation • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis |
Biochem/physiol Actions: | Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations. |
Other Notes: | In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H315 - H319 |
Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
Hazard Codes | Xi |
Risk Statements | 41 |
Safety Statements | 26-39 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Density | 1.01 g/mL at 20 °C |
UNSPSC | 12161902 |