4-Nitrophenyl phosphate disodium salt hexahydrate
SIGMA/71768 - suitable for enzyme immunoassay, ≥99.0% (enzymatic)
Synonym: p-Nitrophenyl phosphate disodium hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; di-Sodium 4-nitrophenyl phosphate; pNPP disodium hexahydrate
CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
assay | ≥99.0% (enzymatic) |
form | powder or crystals |
impurities | ≤0.1% free nitrophenol |
InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7 |
InChI key | KAKKHKRHCKCAGH-UHFFFAOYSA |
Quality Level | 100 |
SMILES string | O.O.O.O.O.O.[Na+].[Na+].[ |
storage temp. | 2-8°C |
technique(s) | enzyme immunoassay: suitable |
Application: | 4-nitrophenyl phosphate disodium salt hexahydrate has been used: • as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity • as substrate to assay acid phosphatase activity • as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate |
General description: | Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.0% (enzymatic) |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |