Nitrilotriacetic acid
SIGMA/72559 - BioUltra, ≥99.0% (T)
Synonym: N,N-
CAS Number: 139-13-9
Empirical Formula (Hill Notation): C6H9NO6
Molecular Weight: 191.14
EC Number: 205-355-7
MDL Number: MFCD00004287
Linear Formula: N(CH2COOH)3
Product Type: Chemical
| λ | 0.1 M in 1 M NaOH |
| anion traces | chloride (Cl-): ≤100 mg/kg |
| assay | ≥99.0% (T) |
| cation traces | Al: ≤20 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤20 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤500 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder |
| ign. residue | ≤0.05% |
| impurities | insoluble matter, passes filter test |
| InChI | 1S/C6H9NO6/c8-4(9)1-7(2-5 |
| InChI key | MGFYIUFZLHCRTH-UHFFFAOYSA |
| loss | ≤0.1% loss on drying, 110 °C |
| mp | 245 °C (dec.) (lit.) |
| pH | 1.7-2.7 (23 °C, 10 g/L) |
| product line | BioUltra |
| Quality Level | 200  |
| reaction suitability | reagent type: reductant |
| SMILES string | OC(=O)CN(CC(O)=O)CC(O)=O |
| solubility | 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: 1.5 |
| λ: 280 nm Amax: 0.05 |
| Application: | An excellent chelating agent. |
| Application: | Nitrilotriacetic has been used in the preparation of nitrilotriacetic (NTA) solution for Ni-NTA slide generation. |
| Biochem/physiol Actions: | Nitrilotriacetic (NTA) has the ability to link with histidine side chain of proteins. It can be used in fluorescent labelling, hexahistidine (His6)-tagged proteins for purification and surface immobilization. |
| Other Notes: | Chelating agent; As Pb2+ buffer, preparation and properties; In a mixture of Ca2+-buffering ligands; Encapsulation of radioactive metal ions in liposomes; Compilation and critical evaluation by IUPAC of published data on stability constants of NTA complexes in solution. |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H319 - H351 |
| Precautionary statements | P201 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 36-40 |
| Safety Statements | 26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | 212.0 °F - closed cup |
| Flash Point(C) | 100 °C - closed cup |
| Purity | ≥99.0% (T) |
| mp | 245 °C (dec.) (lit.) |
| UNSPSC | 12352102 |