Tris(2-carboxyethyl)phosphine hydrochloride
SIGMA/75259 - BioUltra, ≥98% (NMR)
Synonym: TCEP
CAS Number: 51805-45-9
Empirical Formula (Hill Notation): C9H15O6P · HCl
Molecular Weight: 286.65
MDL Number: MFCD00145469
Linear Formula: C9H15O6P · HCl
Product Type: Chemical
λ | 0.1 M in H2O |
anion traces | sulfate (SO42-): ≤50 mg/kg |
assay | ≥98% (NMR) |
cation traces | Al: ≤5 mg/kg |
As: ≤0.05 mg/kg | |
Ba: ≤5 mg/kg | |
Bi: ≤5 mg/kg | |
Ca: ≤10 mg/kg | |
Cd: ≤5 mg/kg | |
Co: ≤5 mg/kg | |
Cr: ≤5 mg/kg | |
Cu: ≤5 mg/kg | |
Fe: ≤5 mg/kg | |
K: ≤50 mg/kg | |
Li: ≤5 mg/kg | |
Mg: ≤5 mg/kg | |
Mn: ≤5 mg/kg | |
Mo: ≤5 mg/kg | |
Na: ≤50 mg/kg | |
Ni: ≤5 mg/kg | |
Pb: ≤5 mg/kg | |
Sr: ≤5 mg/kg | |
Zn: ≤5 mg/kg | |
form | crystals |
InChI | 1S/C9H15O6P.ClH/c10-7(11) |
InChI key | PBVAJRFEEOIAGW-UHFFFAOYSA |
loss | ≤1.0% loss on drying |
pH | 1.2-1.5 |
product line | BioUltra |
Quality Level | 200 |
reaction suitability | reagent type: reductant |
SMILES string | Cl[H].OC(=O)CCP(CCC(O)=O) |
solubility | H2O: 1 M, clear, colorless (no residue) |
storage temp. | 2-8°C |
UV absorption | λ: 260 nm Amax: 0.05 |
λ: 280 nm Amax: 0.03 |
Application: | Tris(2-carboxyethyl)phosp • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA • in reducing sulfhydryl groups to obtain total thiol fraction • to reduce protein samples |
Application: | Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications. |
General description: | Tris(2-carboxyethyl)-phos |
Symbol | GHS05 |
Signal word | Danger |
Hazard statements | H314 |
Precautionary statements | P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
Hazard Codes | C |
Risk Statements | 34 |
Safety Statements | 26-27-36/37/39 |
RIDADR | UN 3261 8 / PGII |
WGK Germany | WGK 1 |
Purity | ≥98% (NMR) |
Storage Temp. | 2-8°C |
UNSPSC | 12352128 |