Osmium tetroxide solution
SIGMA/75632 - suitable for electron microscopy, 4% in H2O
Synonym: Osmium(IV) oxide
CAS Number: 20816-12-0
Empirical Formula (Hill Notation): O4Os
Molecular Weight: 254.23
MDL Number: MFCD00011150
Linear Formula: OsO4
Product Type: Chemical
| application(s) | hematology histology |
| color | colorless to yellow |
| concentration | 4% in H2O |
| density | 1.0 g/mL at 20 °C |
| form | solution |
| InChI | 1S/4O.Os |
| InChI key | VUVGYHUDAICLFK-UHFFFAOYSA |
| pH | 6.0-8.0 |
| Quality Level | 100  |
| reaction suitability | reagent type: oxidant |
| SMILES string | O=[Os](=O)(=O)=O |
| storage temp. | 2-8°C |
| technique(s) | electron microscopy: suitable |
| Application: | Osmium tetroxide solution has been used as a fixative in scanning and transmission electron microscopy. |
| Biochem/physiol Actions: | Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining. |
| Other Notes: | Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical. |
| Packaging: | 5 mL in ampule 10 mL in ampule |
| Symbol |   GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H302 + H332 - H310 - H315 - H318 |
| Precautionary statements | P262 - P280 - P301 + P312 - P302 + P352 + P310 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25 |
| Safety Statements | 36/37-45 |
| RIDADR | UN 3287 6.1 / PGII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.0 g/mL at 20 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |