Peroxidase from horseradish
SIGMA/77332 - lyophilized, powder, ~150 U/mg
Synonym: (2R,3S,6S,7R,8R)
CAS Number: 9003-99-0
EC Number: 232-668-6
MDL Number: MFCD00071339
Product Type: Chemical
| biological source | horseradish |
| form | powder |
| InChI | 1S/H2O3/c1-3-2/h1-2H |
| InChI key | JSPLKZUTYZBBKA-UHFFFAOYSA |
| mol wt | Mr ~40000 |
| quality | lyophilized |
| Quality Level | 100  |
| shipped in | wet ice |
| solubility | 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA) |
| specific activity | ~150 U/mg |
| storage temp. | 2-8°C |
| Application: | Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry. |
| Application: | Peroxidase from horseradish has been used: • for isolation of N-Linked glycans • as a constituent of D-amino acid oxidase (DAAO) buffer • to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS) |
| General description: | Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays. |
| Other Notes: | Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater |
| Unit Definition: | 1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 - H412 |
| Precautionary statements | P261 - P273 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~150 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |