Phenol
SIGMA/77607 - BioUltra, for molecular biology, TE-saturated, ~73% (T)
Synonym: Hydroxybenzene
CAS Number: 108-95-2
Empirical Formula (Hill Notation): C6H6O
Molecular Weight: 94.11
EC Number: 203-632-7
MDL Number: MFCD00002143
Linear Formula: C6H5OH
Product Type: Chemical
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| assay | ~73% (T) |
| autoignition temp. | 1319 °F |
| bp | 182 °C (lit.) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| contains | ~0.1% 8-hydroxyquinoline as preservative |
| density | 1.071 g/mL at 25 °C (lit.) |
| expl. lim. | 8.6 % |
| form | liquid |
| grade | for molecular biology |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C6H6O/c7-6-4-2-1-3-5-6 |
| InChI key | ISWSIDIOOBJBQZ-UHFFFAOYSA |
| mp | 40-42 °C (lit.) |
| pH | ~7.8(aqueous phase) |
| product line | BioUltra |
| quality | TE-saturated |
| Quality Level | 100  |
| refractive index | n |
| SMILES string | Oc1ccccc1 |
| storage temp. | 2-8°C |
| suitability | suitable for molecular biology |
| vapor density | 3.24 (vs air) |
| vapor pressure | 0.09 psi ( 55 °C) |
| 0.36 mmHg ( 20 °C) |
| Application: | Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation. It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis. |
| Caution: | according to temperature, a small quantity of an overlaid aqueous phase may form |
| General description: | Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically. It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning. |
| General description: | saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA |
| Other Notes: | RNA fingerprinting - preparation of the E. coli tRNA carrier |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H311 + H331 - H314 - H341 - H373 - H411 |
| Precautionary statements | P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25-34-48/20/21/22-68 |
| Safety Statements | 24/25-26-28-36/37/39-45 |
| RIDADR | UN 2821 6.1 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 174.2 °F - closed cup |
| Flash Point(C) | 79 °C - closed cup |
| Purity | ~73% (T) |
| bp | 182 °C (lit.) |
| mp | 40-42 °C (lit.) |
| Density | 1.071 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352104 |