Phenol – chloroform – isoamyl alcohol mixture

SIGMA/77618 - BioUltra, for molecular biology, 49.5:49.5:1

Synonym: phenol:Chloroform:Isoamyl Alcohol

CAS Number: 136112-00-0
MDL Number: MFCD00133763
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-77618-100ML	100 mL	$96.20 1/EA		Add To Favorites
45-77618-500ML	500 mL	$347.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

absorption	cut-off at 360 nm
density	1.29 g/mL at 20 °C
form	liquid
grade	for molecular biology
impurities	DNases, none detected
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key	ZYWFEOZQIUMEGL-UHFFFAOYSA-N
pH	7.7-8.3(H₂O-phase, after extraction with H₂O, 1:1)
product line	BioUltra
Quality Level	100
SMILES string	ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
storage temp.	2-8°C

Application:	Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022 ). Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018 ). The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015 ). The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013 ).
Other Notes:	Large- and small-scale phenol extractions
Physical form:	mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Symbol	GHS05,GHS06,GHS08,GHS09
Signal word	Danger
Hazard statements	H301 - H312 + H332 - H314 - H336 - H341 - H351 - H361d - H372 - H373 - H411
Precautionary statements	P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes	T
Risk Statements	63-23/24/25-34-40-48/20/21/22-68
Safety Statements	26-36/37/39-45
RIDADR	UN2810 - class 6.1 - PG 2 - EHS - Toxic, liquids, organic, n.o.s
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.29 g/mL at 20 °C
Storage Temp.	2-8°C
UNSPSC	12352401

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