Atto 490LS maleimide
SIGMA/78363 - suitable for fluorescence
Product Type: Chemical
| λ | in PBS, pH 7.4 |
| assay | ≥90% (HPLC) |
| fluorescence | λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4 |
| form | (Report result) |
| manufacturer/tradename | ATTO-TEC GmbH |
| mol wt | Mw 818 g/mol |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| transmittance | 254 nm, >90% |
| 490 nm, >90% | |
| UV absorption | λ: 494-500 nm Amax |
| General description: | Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols. find more information here |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352111 |