Lipase from Rhizopus oryzae
SIGMA/80612 - powder, light brown, ≥30 U/mg
Synonym: Lipase F-AP 15
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
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| biological source | fungus (Rhizopus oryzae) |
| color | light brown |
| form | powder |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| specific activity | ≥30 U/mg |
| storage temp. | 2-8°C |
| Analysis Note: | enzyme activity: the optimum temperature is 40°C, the optimum pH is 7.2 (highly active from pH 6.5-7.5) |
| Application: | Lipase from Rhizopus oryzae has been used: • to replace rabbit gastric extract for infant in vitro digestion • in the two-phase lipase-catalyzed system for the enzymatic preparation of biodiesel • in the simulation of infant gastrointestinal digestion in vitro, focusing on the characteristics of emulsions during digestion to replicate the stomach environment and adjust the emulsion pH |
| Biochem/physiol Actions: | Lipases play a crucial role in catalyzing the breakdown of ester bonds of triglycerides at the interface between aqueous and oily layers. The lipase produced by Rhizopus species is known for its suitability in various industrial applications. In industrial settings, lipases of microbial origin are commonly extracellular and are produced during the fermentation process. The utilization of lipases has been shown to be more effective than traditional chemical syntheses in industries such as pharmaceuticals, cosmetics, oleochemicals, detergents, and fragrances. These enzymes are widely used to modify the structure of oils and fats, tailoring natural lipids to meet specific properties useful for food, nutrition, and cosmetic applications. Lipases from filamentous fungi families, such as Rhizopus oryzae are extensively utilized in the oil and fats industry due to their high 1,3-regioselectivity toward triglycerides, making them versatile in lipid modification. Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| General description: | Research Area: CELL SIGNALING,Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a 26-amino acid presequence, followed by a 97-amino acid prosequence attached to the N-terminal of a mature sequence consisting of 269 amino acids. ROL possesses four potential N-glycosylation sites and is characterized by nine α-helices and eight β-strands, resulting in a molecule stabilized by three disulfide bonds between residues 29–269, 40–43, and 235–244. Lipases are widely distributed in various living organisms, including animals, plants, and microbes. |
| Other Notes: | 1 U corresponds to the amount of enzyme which releases 1 μmol fatty acid from triglycerides per minute at pH 7.2 and 37 C (olive oil as substrate) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3  ( BRENDA  | IUBMB ); 3.1.1.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |