Rhodamine B
SIGMA/83689 - suitable for fluorescence
Synonym: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine
CAS Number: 81-88-9
Empirical Formula (Hill Notation): C28H31ClN2O3
Molecular Weight: 479.01
EC Number: 201-383-9
MDL Number: MFCD00011931
Linear Formula: C28H31ClN2O3
Product Type: Chemical
| color | green to very dark green |
| concentration | 80-100 % (w/w) (Rhodamine B) |
| density | 0.79 g/cm3 |
| fluorescence | λex 553 nm; λem 627 nm in methanol |
| form | powder or crystals |
| InChI | 1S/C28H30N2O3.ClH/c1-5-29 |
| InChI key | PYWVYCXTNDRMGF-UHFFFAOYSA |
| mol wt | 479.01 g/mol |
| mp | 210-211 (dec.) (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | [Cl-].CCN(CC)c1ccc2c(OC3= |
| solubility | H2O: soluble |
| methanol: soluble | |
| suitability | in accordance for fluorescence |
| suitable for fluorescence |
| Analysis Note: | λmax. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol) |
| Application: | for cross staining of N-methylamino-5-isocyanon |
| Application: | Rhodamine B has been used: • as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanona • as a fluorescent dye in confocal microscopy • to study its fluorescence in water and ethanol solutions |
| General description: | Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye. |
| General description: | Rhodamine B is bright-red in color. |
| Symbol | ![]() GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H318 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 + P330 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn |
| Risk Statements | 22-41-52/53 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 210-211 (dec.) (lit.) |
| Density | 0.79 g/cm3 |
| Colour Index Number | 45170 |
| UNSPSC | 12171500 |



