Rhodamine 123
SIGMA/83702 - BioReagent, for fluorescence, ≥85% (HPLC)
Synonym: 2-
CAS Number: 62669-70-9
Empirical Formula (Hill Notation): C21H16N2O3 · HCl
Molecular Weight: 380.82
EC Number: 263-687-8
MDL Number: MFCD00012664
Linear Formula: C21H17ClN2O3
Product Type: Chemical
| assay | ≥85% (HPLC) |
| form | powder |
| grade | for fluorescence |
| impurities | ≤10% water |
| InChI | 1S/C21H16N2O3.ClH/c1-25-2 |
| InChI key | MYFATKRONKHHQL-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | Cl[H].COC(=O)c1ccccc1C2=C |
| solubility | ethanol: 20 mg/mL, clear, red |
| suitability | in accordance for fluorescence |
| Application: | Rhodamine 123 has been used: • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays • to label the cells for estimating the P-gp expression by flow cytometry • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport) • the detection of mitochondrial membrane potential (ΔΨm) |
| Biochem/physiol Actions: | Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin |
| Biochem/physiol Actions: | Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry. |
| General description: | Rhodamine 123 is a fluorescent dye. |
| Other Notes: | Supravital mitochondrial-specific dye |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (HPLC) |
| UNSPSC | 12352116 |