Safranin O
SIGMA/84120 - for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)
Synonym: Basic Red 2; Cotton Red; Gossypimine; Safranin T; Safranin Y or A
CAS Number: 477-73-6
Empirical Formula (Hill Notation): C20H19ClN4
Molecular Weight: 350.84
EC Number: 207-518-8
MDL Number: MFCD00011759
Linear Formula: C20H19ClN4
Product Type: Chemical
| εmax | ≥1000 at 525-535 nm in 50% ethanol |
| application(s) | diagnostic assay manufacturing hematology histology microbiology |
| form | powder or crystals |
| grade | for microscopy (Bact., Bot., Hist.) |
| indicator (pH 0.3-1.0) | |
| InChI | 1S/C20H18N4.ClH/c1-12-8-1 |
| InChI key | OARRHUQTFTUEOS-UHFFFAOYSA |
| pH | 10 (20 °C, 10 g/L) |
| Quality Level | 200  |
| SMILES string | [Cl-].Cc1cc2nc3cc(C)c(N)c |
| solubility | methanol: 0.01 g/10 mL, red |
| storage temp. | room temp |
| technique(s) | titration: suitable |
| Application: | • Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores. • It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei. • It is a component of Benda′s polychrome stain. • It is a component of various polychromes and lignin stains for botanical staining applications. • It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections. |
| General description: | Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | room temp |
| Colour Index Number | 50240 |
| UNSPSC | 12171500 |