Streptavidin from Streptomyces avidinii
SIGMA/85878 - affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein
CAS Number: 9013-20-1
MDL Number: MFCD00082035
Product Type: Chemical
ELISA assay utilizing a biotinylated antibody and streptavidin-peroxidase detection.
HABA Assay HABA is a dye with absorbance and Avidin-binding properties. When HABA is bound to Avidin, the complex absorbs light at 500 nm. When a biotin sample is added, the biotin binds strongly to Avidin and displaces the weakly bound HABA resulting in a decrease in absorbance relative to the amount of HABA displaced.
| form | powder |
| mol wt | Mr ~60000 |
| quality | affinity purified |
| lyophilized from 10 mM potassium phosphate | |
| Quality Level | 200  |
| solubility | H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow |
| specific activity | ≥13 U/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied. |
| Application: | Streptavidin from Streptomyces avidinii has been used: • in the pre-functionalization of self-propelled catalytic micromotors • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC) |
| Biochem/physiol Actions: | Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization. |
| General description: | Streptavidin is a crystalline protein synthesized by Streptomycetes. |
| Other Notes: | Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology |
| Packaging: | 5, 10 mg in glass bottle |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Packaging: | The sales quantitites 1 mg and 5 mg |
| Unit Definition: | 1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥13 U/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |