5-Carboxyfluorescein
SIGMA/86826 - BioReagent, suitable for fluorescence, ≥95% (HPLC)
Synonym: 5-FAM
CAS Number: 76823-03-5
Empirical Formula (Hill Notation): C21H12O7
Molecular Weight: 376.32
MDL Number: MFCD00036874
Linear Formula: C21H12O7
Product Type: Chemical
assay | ≥95% (HPLC) |
fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 |
form | powder |
InChI | 1S/C21H12O7/c22-11-2-5-15 |
InChI key | NJYVEMPWNAYQQN-UHFFFAOYSA |
product line | BioReagent |
Quality Level | 100 |
SMILES string | OC(=O)c1ccc2c(c1)C(=O)OC2 |
solubility | DMF: soluble |
DMSO: soluble | |
H2O: soluble | |
suitability | suitable for fluorescence |
Application: | 5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes. |
General description: | 5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane. |
Other Notes: | Derivative of fluorescein giving stable derivative upon conjugation with biopolymers |
Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC) |
UNSPSC | 12352200 |