Chang Liver (HeLa derivative) cell line
SIGMA/88021102
Synonym: Chang Liver cell line (HeLa derivative)
Product Type: Chemical
| biological source | human cervix |
| form | liquid |
| growth mode | Adherent |
| karyotype | Modal no. 70 |
| morphology | Epithelial |
| products | Not specified |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Chang Liver (HeLa derivative) cell line has been used to study the construction and biological activity of human torque teno virus (TTV) genotype 6. |
| Application: | Virus, biochemical and replication studies |
| Cell Line Description: | The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black. |
| Cell Line Origin: | Human cervix carcinoma |
| Culture Medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 12,14 D16S539: 9,10 D5S818: 12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |