BER human cell line
SIGMA/8805209 - IHW Number: 9093
Product Type: Chemical
| biological source | human blood |
| ethnicity | German |
| form | liquid |
| gender | male |
| growth mode | Suspension |
| karyotype | Not specified |
| morphology | Lymphoblastoid |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −196°C |
| Cell Line Description: | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| Culture Medium: | RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS ) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| Disclaimer: | This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations. |
| DNA Profile: | A: 201 Cw: 602 B: 1302 HLA DRA: 101 HLA DRB1: 701 HLA DRB4: 101 HLA DQAP: 2.1 HLA DQA1: 201 HLA DQB1: 201 HLA DPA1: 1 HLA DPB1: 02012 0401 |
| General description: | Consanguineous: No |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Serological Profile: | HLA A: 2 HLA A ief: 2.2 HLA C: 6 HLA B: 13 HLA B ief: 13.2 Bw4/bw6: 4 HLA Dw: 7 HLA DR: 7 HLA DR53: 53 HLA DQ: 2 HLA DP: 2 4 |
| Subculture Routine: | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |