Atto 532 NHS ester
SIGMA/88793 - BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym: Atto 532
MDL Number: MFCD05865401
Product Type: Chemical
λ | in methanol: water (1:1) (with 0.1% perchloric acid) |
assay | ≥90% (degree of coupling) |
≥90% (HPLC) | |
form | powder |
manufacturer/tradename | ATTO-TEC GmbH |
product line | BioReagent |
Quality Level | 100 |
storage temp. | −20°C |
suitability | suitable for fluorescence |
UV absorption | λ: 532-538 nm Amax |
Application: | Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods. |
Features and Benefits: | Characteristic features of the Atto 532 NHS ester are: • Strong Absorption. • High Fluorescence quantum yield. • High Photostability. • Excellent water solubility. |
General description: | Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90% (HPLC); ≥90% (degree of coupling) |
Storage Temp. | −20°C |
UNSPSC | 12352108 |