Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit
SIGMA/90A1 - Select reagents packaged in gelatin capsules.
MDL Number: MFCD08705438
Product Type: Chemical
Staining image is the result of Procedure No. 90.
α-Naphthyl Acetate (Non-Specific Esterase) is intended for in vitro diagnostic use. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| application(s) | hematology histology |
| form | liquid |
| IVD | for in vitro diagnostic use |
| Quality Level | 500  |
| shelf life | Expiry date on the label. |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing. |
| Application: | Intended for the cytologic demonstration of a-naphthyl acetate esterase in blood, bone marrow films or tissue touch preparations. |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 + H312 + H332 - H314 - H360FD - H370 - H373 |
| Precautionary statements | P201 - P210 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P311 |
| Hazard Codes | T |
| Risk Statements | 60-61-10-20/21/22-34 |
| Safety Statements | 53-26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 104.0 °F - closed cup |
| Flash Point(C) | 40 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 41116123 |
| Components | α-Naphthyl acetate (90-6) 10 x 20 mg; Citrate solution (386-1) 20 mL; Ethylene glycol monomethyl ether 25 mL; Fast Blue RR Salt (FBS-25) 10 caps; Mayer's Hematoxylin Solution (MHS-1) 100 mL; TRIZMAL™ 7.6 Concentrate 50 mL |