Insulin, Human Recombinant
SIGMA/91077C - dry powder, for research or for further manufacturing use
Synonym: Insulin sodium salt human; rh-Insulin
CAS Number: 11061-68-0
EC Number: 234-279-7
MDL Number: MFCD00131380
Product Type: Chemical
| form | dry powder |
| impurities | ≤1.0 EU/mL endotoxin |
| InChI key | PBGKTOXHQIOBKM-FHFVDXKLSA |
| potency | ≥27.5 units per mg |
| Quality Level | 300  |
| shipped in | ambient |
| solubility | water: 10 mg/mL (pH 2.0—2.5) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01308  |
| Application: | Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate. • Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells. • It has been used as a supplement in the cell culture media of airway epithelial cells. • It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance. |
| Biochem/physiol Actions: | Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. |
| General description: | Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | InChI: 1S/C257H383N65O77S6/c1-29 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |