Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit
SIGMA/91A - Kit formulated with all liquid reagents.
MDL Number: MFCD08705438
Product Type: Chemical
application(s) | hematology histology |
form | liquid |
IVD | for in vitro diagnostic use |
Quality Level | 500 |
shelf life | Expiry date on the label. |
shipped in | wet ice |
storage temp. | 2-8°C |
Application: | Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing. |
Symbol | GHS02,GHS05,GHS06,GHS08 |
Signal word | Danger |
Hazard statements | H226 - H290 - H301 - H312 + H332 - H319 - H370 - H373 |
Precautionary statements | P210 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | T |
Risk Statements | 10-23/24/25-37/38-39/23/24/25-41-42/43 |
Safety Statements | 23-26-36/37/39-45 |
RIDADR | UN 3316 9 |
WGK Germany | WGK 3 |
Flash Point(F) | 98.1 °F |
Flash Point(C) | 36.7 °C |
Storage Temp. | 2-8°C |
UNSPSC | 41116123 |
Components | α-Naphthyl Acetate Solution 10 mL; Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL; Fast Blue BB Base Solution 10 mL; Sodium nitrite solution 10 mL; TRIZMAL™ 7.6 Concentrate 50 mL; Hematoxylin solution gill no. 3 50 mL |