Leukocyte Naphthol AS-D Chloroacetate (Specific Esterase) Kit
SIGMA/91C
Synonym: Leder Stain
Product Type: Chemical
Leder stain
| application(s) | hematology histology |
| description | Kit formulated with all liquid reagents |
| IVD | for in vitro diagnostic use |
| Quality Level | 500  |
| shelf life | Expiry date on the label. |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 91C kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium compound, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded. |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H290 - H302 - H315 - H317 - H318 - H335 - H360FD - H373 |
| Precautionary statements | P202 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | C |
| Risk Statements | 19-35 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 2 |
| Supplemental Hazard Statements | EUH019 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL; Fast Red Violet LB Base Solution 10 mL; Naphthol AS-D Chloroacetate Solution 10 mL; Naphthol AS-D Chloroacetate Solution 50 mL |