TRI Reagent®
SIGMA/93289 - for DNA, RNA and protein isolation
Synonym: DNA/RNA/protein extraction reagent; DNA/RNA/protein isolation reagent; DNA/RNA/protein purification reagent; single step RNA extraction reagent; total RNA extraction reagent; TRI Reagent® RNA Isolation Reagent
MDL Number: MFCD00213058
Product Type: Chemical
| form | liquid |
| grade | for DNA, RNA and protein isolation |
| impurities | phenol |
| Quality Level | 100  |
| usage | mL sufficient for 100 mg tissue |
| mL sufficient for 107 cells |
| Application: | TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin. |
| Application: | TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets. |
| Features and Benefits: | • Easily scalable RNA isolation • Works with many sources: human, plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate/cesium chloride methods |
| General description: | TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells. |
| Legal Information: | TRI Reagent is a registered trademark of Molecular Research Center, Inc. |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H311 + H331 - H314 - H341 - H373 - H411 |
| Precautionary statements | P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25-32-34-48/20/21/22-52/53-68 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN 2922 6.1(8) / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 174.2 °F - closed cup |
| Flash Point(C) | 79 °C - closed cup |
| Supplemental Hazard Statements | EUH032 |
| UNSPSC | 12352200 |