Trizma® maleate
SIGMA/93328 - BioUltra, ≥99.5% (NT)
Synonym: TRIS maleate salt; Tris(hydroxymethyl)
CAS Number: 72200-76-1
Empirical Formula (Hill Notation): C4H11NO3 · C4H4O4
Molecular Weight: 237.21
EC Number: 276-455-6
MDL Number: MFCD00082442
Linear Formula: NH2C(CH2OH)3 · C4H4O4
Product Type: Chemical
absorption | cut-off at 328 nm in H2O at 0.5 M |
anion traces | chloride (Cl-): ≤50 mg/kg |
sulfate (SO42-): ≤50 mg/kg | |
assay | ≥99.5% (NT) |
cation traces | Al: ≤5 mg/kg |
As: ≤0.1 mg/kg | |
Ba: ≤5 mg/kg | |
Bi: ≤5 mg/kg | |
Ca: ≤10 mg/kg | |
Cd: ≤5 mg/kg | |
Co: ≤5 mg/kg | |
Cr: ≤5 mg/kg | |
Cu: ≤5 mg/kg | |
Fe: ≤5 mg/kg | |
K: ≤50 mg/kg | |
Li: ≤5 mg/kg | |
Mg: ≤5 mg/kg | |
Mn: ≤5 mg/kg | |
Mo: ≤5 mg/kg | |
Na: ≤50 mg/kg | |
Ni: ≤5 mg/kg | |
Pb: ≤5 mg/kg | |
Sr: ≤5 mg/kg | |
Zn: ≤5 mg/kg | |
form | powder or crystals |
ign. residue (900 °C) | ≤0.1% (as SO4) |
impurities | insoluble matter, passes filter test |
InChI | 1S/C4H11NO3.C4H4O4/c5-4(1 |
InChI key | HTMWOUBCEZXSHN-BTJKTKAUSA |
pH | 3.0-4.5 (25 °C, 0.5 M in H2O) |
product line | BioUltra |
Quality Level | 100 |
SMILES string | NC(CO)(CO)CO.OC(=O)C=C/C |
solubility | H2O: 0.5 M at 20 °C, clear, colorless |
Application: | Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma maleate is used in the preparation of Tris maleate buffers with lower effective pH buffering capacity than Tris buffers. Tris maleate buffers may be used in various grow media, in tissue preparations and as a diluent or washing buffer. |
Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.5% (NT) |
UNSPSC | 12161700 |