Trizma® acetate
SIGMA/93337 - BioUltra, ≥99.0% (NT)
Synonym: TRIS acetate salt; Tris(hydroxymethyl)
CAS Number: 6850-28-8
Empirical Formula (Hill Notation): C4H11NO3 · CH3COOH
Molecular Weight: 181.19
EC Number: 229-939-6
MDL Number: MFCD00038951
Linear Formula: NH2C(CH2OH)3 · CH3COOH
Product Type: Chemical
| λ | 0.5 M in H2O |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.0% (NT) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | crystalline powder |
| ign. residue | ≤0.2% |
| impurities | insoluble matter, passes filter test |
| InChI | 1S/C4H11NO3.C2H4O2/c5-4(1 |
| InChI key | PIEPQKCYPFFYMG-UHFFFAOYSA |
| loss | ≤0.2% loss on drying, 20 °C (HV) |
| pH | 6.0-7.0 (25 °C, 0.5 M in H2O) |
| product line | BioUltra |
| Quality Level | 300  |
| SMILES string | CC(O)=O.NC(CO)(CO)CO |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: 0.07 |
| λ: 280 nm Amax: 0.05 |
| Application: | Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (NT) |
| UNSPSC | 12161700 |