Trizma® hydrochloride
SIGMA/93363 - BioUltra, for molecular biology, ≥99.0% (AT)
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
EC Number: 214-684-5
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
| λ | 4 M in H2O |
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| assay | ≥99.0% (AT) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder or crystals |
| grade | for molecular biology |
| ign. residue | ≤0.2% (as SO4) |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| loss | ≤0.2% loss on drying, 110 °C |
| mp | 150-152 °C |
| pH | 2.5-4.0 (25 °C, 4 M in H2O) |
| pKa (25 °C) | 8.1 |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | Cl.NC(CO)(CO)CO |
| solubility | H2O: 4 M at 20 °C, clear, colorless |
| useful pH range | 7.0-9.0 |
| UV absorption | λ: 260 nm Amax: 0.12 |
| λ: 280 nm Amax: 0.08 |
| Application: | Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE. |
| Other Notes: | Easily compare specifications for Trizma HCl products with the Trizma HCl specification table . |
| Other Notes: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (AT) |
| mp | 150-152 °C |
| UNSPSC | 12161700 |