Triton™ X-100 solution
SIGMA/93443 - BioUltra, for molecular biology, ~10% in H2O
Synonym: Triton™ X-100; 4-
CAS Number: 9036-19-5
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical
| absorption | cut-off at 310 nm |
| aggregation number | 100-155 |
| cation traces | Al: ≤1 mg/kg |
| Ba: ≤1 mg/kg | |
| Bi: ≤1 mg/kg | |
| Ca: ≤5 mg/kg | |
| Cd: ≤1 mg/kg | |
| Co: ≤1 mg/kg | |
| Cr: ≤1 mg/kg | |
| Cu: ≤1 mg/kg | |
| Fe: ≤1 mg/kg | |
| K: ≤100 mg/kg | |
| Li: ≤1 mg/kg | |
| Mg: ≤1 mg/kg | |
| Mn: ≤1 mg/kg | |
| Mo: ≤1 mg/kg | |
| Na: ≤100 mg/kg | |
| Ni: ≤1 mg/kg | |
| Pb: ≤1 mg/kg | |
| Sr: ≤1 mg/kg | |
| Zn: ≤1 mg/kg | |
| CMC | 0.2-0.9 mM (20-25°C) |
| 0.23 mM | |
| concentration | ~10% in H2O |
| density | 1.01 g/mL at 20 °C |
| 1.070 g/cm3 | |
| description | non-ionic |
| form | liquid |
| grade | for molecular biology |
| HLB | 13.5 |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C28H50O8/c1-27(2,3)24- |
| InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA |
| mol wt | average mol wt 625 |
| micellar avg mol wt 80,000 | |
| pH | 6.5-8.5 (25 °C) |
| product line | BioUltra |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCC |
| transition temp | cloud point 65 °C |
| pour point ~7 °C |
| Application: | Triton™ X-100 has been used:• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry • As a component of lysis buffer used for homogenization of cells • As a component of blocking buffer in immunofluorescence staining • For the preparation of cells in double fluorescence staining |
| Biochem/physiol Actions: | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. |
| Features and Benefits: | • Suitable for Molecular Biology and Biochemical Research • Free from DNase, RNase, NICKase and protease • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications. • High purity product for research applications • Tested for the presence of Anionic and Cationic traces |
| General description: | Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Symbol |   GHS05,GHS09 |
| Signal word | Danger |
| Hazard statements | H315 - H318 - H410 |
| Precautionary statements | P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310 |
| Risk Statements | 52/53 |
| Safety Statements | 60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | 483.8 °F - closed cup |
| Flash Point(C) | 251 °C - closed cup |
| Density | 1.01 g/mL at 20 °C; 1.070 g/cm3 |
| Refractive Index | n |
| UNSPSC | 12161900 |