Trypsin inhibitor from Glycine max (soybean)
SIGMA/93620 - lyophilized powder, ~10000 U/mg
Synonym: SBTI
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082103
Product Type: Chemical
Trypsin Inhibitor (Kunitz) (soybean)
| biological source | Glycine max (soybean) |
| form | lyophilized powder |
| mol wt | Mr ~20000 |
| Quality Level | 100  |
| shipped in | wet ice |
| solubility | 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless |
| specific activity | ~10000 U/mg |
| storage temp. | −20°C |
| Biochem/physiol Actions: | This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent. |
| Other Notes: | 1 U corresponds to the amount of inhibitor which reduces the trypsin activity by 1 BAEE-U. (1 BAEE-U is the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25 °C; BAEE, Cat. No. 12880, as substrate). |
| Other Notes: | For the inhibition of proteolytic activity; Use in affinity chromatography for the purification of trypsin; Prepared by the method of M. Kunitz. |
| Other Notes: | One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginin |
| Preparation Note: | The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ~10000 U/mg |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |