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Atto 655

SIGMA/93711 - BioReagent, suitable for fluorescence, ≥85% (HPLC)

MDL Number: MFCD03456136
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-93711-1MG-F 1 mg
$279.00
1/EA
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λ in ethanol (with 0.1% trifluoroacetic acid)
assay ≥85% (HPLC)
fluorescence λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
form powder
InChI 1S/C27H33N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-14,18H,4-10,15-16H2,1-3H3,(H-,31,32,33,34,35)
InChI key FOYVTVSSAMSORJ-UHFFFAOYSA-N
manufacturer/tradename ATTO-TEC GmbH
product line BioReagent
Quality Level 100 
storage temp. −20°C
suitability suitable for fluorescence
transmittance 254 nm
  655 nm
UV absorption λ: 652-658 nm Amax
Application: Atto labels are designed for highest sensitivity applications. A unique combination of advantages makes them highly favorable tools for all kinds of labeling applications. Some of their properties make them specifically interesting for single molecule detection. Atto labels are based on rigid structures and do not show any cis-trans-isomerization, which lowers the brightness of signals and leads to environment dependency, e.g., spectral shifts by conjugation.
Atto 655 shows a molar extinction of 110,000 and QY of 30% in water (50% in ethanol). Decay time is 1.9 ns.
Other Notes: New red absorbing fluorescent dye with best signal-to-noise ratio and long fluorescence life-time. Useful as a biophysical probe for binding interactions.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥85% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

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