TWEEN® 20
SIGMA/93773 - viscosity 250-450 mPa.s (25 °C)
Synonym: Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL Number: MFCD00165986
Product Type: Chemical
| density | 1.095 g/mL at 25 °C (lit.) |
| 1.11 g/mL at 20 °C | |
| description | non-ionic |
| form | viscous liquid |
| HLB value | 16.7±1.0 |
| InChI | 1S/C26H50O10/c1-2-3-4-5-6 |
| InChI key | HMFKFHLTUCJZJO-UHFFFAOYSA |
| mol wt | ~1228 |
| Quality Level | 200  |
| refractive index | n |
| n |
|
| SMILES string | CCCCCCCCCCCC(=O)OCCOCC(C1 |
| technique(s) | cell culture | mammalian: suitable |
| DNA extraction: suitable | |
| protein purification: suitable | |
| RNA extraction: suitable | |
| viscosity | 250-450 mPa.s(25 °C) |
| Application: | Non-ionic detergent |
| Application: | Tween® 20 is widely used in several biochemical applications. It has been used: • as an emulsifying agent for the preparation of stable oil-in-water emulsions • in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins) • as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1% • for lysing mammalian cells at a concentration of 0.005 to 0.5% • for rehydrating tissue before in situ hybridization |
| Biochem/physiol Actions: | Tween® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic. |
| Caution: | may get turbid on storage, heat to clear |
| Features and Benefits: | • Suitable for protein purification and DNA and RNA extraction • Suitable for mammalian cell culture • High purity product for research applications |
| General description: | Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 527.0 °F - Pensky-Martens c |
| Flash Point(C) | 275 °C - Pensky-Martens clo |
| Density | 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12161900 |