Nitrotetrazolium Blue chloride
SIGMA/93862 - 90.0-110.0% (calc. on dry substance, T)
Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
CAS Number: 298-83-9
Empirical Formula (Hill Notation): C40H30Cl2N10O6
Molecular Weight: 817.64
MDL Number: MFCD00012159
Linear Formula: C40H30N10O6 · 2Cl
Product Type: Chemical
λ | in methanol |
assay | 90.0-110.0% (calc. on dry substance, T) |
form | powder |
impurities | ≤10% solvent content |
≤10% water | |
InChI | 1S/C40H30N10O6.2ClH/c1-55 |
InChI key | FSVCQIDHPKZJSO-UHFFFAOYSA |
Quality Level | 100 |
SMILES string | [Cl-].[Cl-].COc1cc(ccc1-[ |
storage temp. | 2-8°C |
UV absorption | λ: 252-262 nm Amax |
Analysis Note: | solubility: 250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow |
Biochem/physiol Actions: | Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained. It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases. NBT is also used for diagnosing microbial infections and phagocytic defects. In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indoly |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H302 - H315 - H319 - H335 |
Precautionary statements | P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | 90.0-110.0% (calc. on dry substance, T) |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |