RPMI 7932 cell line
SIGMA/94072246 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072246, human lung effusion, Epithelial-like morphology
Product Type: Chemical
| biological source | human lung (effusion) |
| form | liquid |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial-like |
| packaging | pkg of vial of cells 94072246-1VL |
| tube of 5 μg 94072246-DNA-5UG | |
| products | Plasminogen activator |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Tumourigenicity studies |
| Biochem/physiol Actions: | Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated. |
| Biochem/physiol Actions: | Human pleural effusion from a patient with malignant melanoma |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Preparation Note: | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |