WAL, FD human cell line
SIGMA/94082228 - IHW Number: 9129
Product Type: Chemical
| biological source | human blood |
| ethnicity | Caucasoid |
| form | liquid |
| growth mode | Suspension |
| karyotype | Not specified |
| morphology | Lymphoblastoid |
| Quality Level | 100  |
| shipped in | dry ice |
| Cell Line Description: | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| Culture Medium: | RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS ) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Disclaimer: | This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations. |
| DNA Profile: | A: 03 B: 07 HLA DRB1: 1501 HLA DRB5: 101 HLA DQAP: 1.2 HLA DQA1: 102 HLA DQB1: 602 HLA DPB1: 401 |
| General description: | Homozygous: Yes |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Serological Profile: | HLA A: 3 HLA B: 7 |
| Subculture Routine: | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |