Atto 647N-Streptavidin
SIGMA/94149 - BioReagent, suitable for fluorescence
Synonym: Streptavidin-Atto 647N
Product Type: Chemical
| λ | in ethanol (with 0.1% trifluoroacetic acid) |
| form | powder |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | corresponds for gel electrophoresis |
| suitable for fluorescence | |
| UV absorption | λ: 650.0-656.0 nm Amax |
| Analysis Note: | dye-to-protein ratio ≥1.5 |
| Application: | Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging. |
| Features and Benefits: | Atto 647N- Streptavidin has the following benefits- • Highly photostable. Strong absorption. • Excellent fluorescent quantum yield. • Highly resistant to ozone degradation. • Minimal triplet formation. |
| General description: | Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161900 |