Urease from Canavalia ensiformis (Jack bean)
SIGMA/94281 - powder, white, ~8 U/mg
Synonym: Jack bean urease; Urea amidohydrolase
CAS Number: 9002-13-5
EC Number: 232-656-0
MDL Number: MFCD00070858
Product Type: Chemical
| biological source | Canavalia ensiformis |
| color | white |
| form | powder |
| mol wt | Mr ~480000 |
| Quality Level | 100  |
| specific activity | ~8 U/mg |
| storage temp. | −20°C |
| Application: | Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions. |
| Biochem/physiol Actions: | Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit . |
| General description: | Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620 |
| General description: | Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants. |
| Unit Definition: | 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ~8 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |