Hoechst 33258 solution

SIGMA/94403 - 1 mg/mL in H₂O, ≥98.0% (HPLC)

Synonym: bisBenzimide H 33258 solution; bisBenzimide H 33258 solution

CAS Number: 23491-45-4
Empirical Formula (Hill Notation): C₂₅H₂₄N₆O · 3HCl
Molecular Weight: 533.88
Linear Formula: C₂₅H₂₄N₆O · 3HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-94403-1ML	1 mL	$201.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98.0% (HPLC)
concentration	1 mg/mL in H₂O
fluorescence	λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
form	liquid
InChI	1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
InChI key	SMNPLAKEGAEPJD-UHFFFAOYSA-N
Quality Level	100
SMILES string	Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
solubility	DMF: soluble
	H₂O: soluble
storage temp.	2-8°C

Application:	Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.
General description:	Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98.0% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12171500

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