Tetramethylrhodamine-5-maleimide

SIGMA/94506 - suitable for fluorescence, ≥85% (HPLC)

CAS Number: 174568-67-3
Empirical Formula (Hill Notation): C₂₈H₂₃N₃O₅
Molecular Weight: 481.50
MDL Number: MFCD01311091
Linear Formula: C₂₈H₂₃N₃O₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-94506-1MG-F	1 mg	$199.00 1/EA		Add To Favorites
45-94506-5MG-F	5 mg	$682.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥85% (HPLC)
fluorescence	λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0
form	powder
InChI	1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)
InChI key	KGFLZYXDJDOIEE-UHFFFAOYSA-N
Quality Level	100
SMILES string	N5(C(=O)C=CC5=O)c1cc(c(cc1)C2=C3CC=C(C=C3Oc4c2ccc(c4)N(C)C)N(C)C)C(=O)O
solubility	DMSO: soluble
	methanol: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	Tetramethylrhodamine-5-maleimide has been used: • to concentrate the eluate and in labeling to create IRE1LDS112C • to derivatize green fluorescent protein (GFP) on Cys48 for experiments with the extrinsic fluorescent probe • to incubate the single solvent exposed cys mutant to perform protein labeling
Other Notes:	Labeling reagent for protein thiols for cytofluorescence
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥85% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352116

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