Hirudin recombinant from yeast
SIGMA/94581 - lyophilized powder, white, >1500 U/vial
CAS Number: 8001-27-2
EC Number: 232-279-1
MDL Number: MFCD00803952
Product Type: Chemical
| biological source | synthetic |
| yeast (Pichia pastoris) | |
| color | white |
| form | lyophilized powder |
| mol wt | Mr ~6963.5 |
| Quality Level | 100  |
| solubility | 0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless |
| specific activity | >1500 U/vial |
| storage temp. | −20°C |
| Application: | Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay. |
| Biochem/physiol Actions: | Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration. |
| Biochem/physiol Actions: | The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. |
| Unit Definition: | 1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >1500 U/vial |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |