Vanadyl ribonucleoside complexes solution
SIGMA/94742 - BioReagent, for molecular biology, 200 mM
Synonym: Ribonucleoside vanadyl complexes
Product Type: Chemical
concentration | 200 mM |
form | liquid |
grade | for molecular biology |
impurities | oxovanadium V |
product line | BioReagent |
Quality Level | 100 |
shipped in | dry ice |
storage temp. | −20°C |
suitability | in accordance for ribonuclease inhibition test |
Application: | Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA. |
Application: | Vanadyl ribonucleoside complexes solution has been used: • as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer • as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes |
General description: | Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases. |
General description: | Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |