HAY, BD human cell line
SIGMA/9482222 - IHW Number: 9123
Product Type: Chemical
| biological source | human blood |
| ethnicity | Australian Aborigine |
| form | liquid |
| gender | male |
| growth mode | Suspension |
| karyotype | Not specified |
| morphology | Lymphoblastoid |
| Quality Level | 100  |
| shipped in | dry ice |
| Cell Line Description: | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| Culture Medium: | RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS ) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| General description: | Consanguineous: No |
| General description: | Homozygous: No |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
| Subculture Routine: | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352207 |