Water
SIGMA/95284 - Molecular Biology, sterile filtered
Synonym: H2O
CAS Number: 7732-18-5
Empirical Formula (Hill Notation): H2O
Molecular Weight: 18.02
EC Number: 231-791-2
MDL Number: MFCD00011332
Linear Formula: H2O
Product Type: Chemical
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| bp | 100 °C (lit.) |
| cation traces | Ca: <10 ppb |
| Mg: <10 ppb | |
| conductivity | 0.0-5 μS/cm at 25 °C |
| density | 1.000 g/mL at 3.98 °C (lit.) |
| form | liquid |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/H2O/h1H2 |
| InChI key | XLYOFNOQVPJJNP-UHFFFAOYSA |
| mp | 0 °C (lit.) |
| pH | 5-7 |
| quality | sterile filtered |
| Quality Level | 100  |
| refractive index | n |
| shelf life | limited shelf life, expiry date on the label |
| SMILES string | O |
| sterility | sterile-filtered |
| technique(s) | FISH: suitable |
| Application: | Water, sterile filtered has been used: • for cloning and plasmid construction. • for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method. • in RNA isolation. • fluorescence in situ hybridization (FISH). • to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA. • for DNA quantification using quantitative polymerase chain reaction (qPCR). |
| General description: | Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| bp | 100 °C (lit.) |
| mp | 0 °C (lit.) |
| Density | 1.000 g/mL at 3.98 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12191602 |