Ascorbate Oxidase from Cucurbita sp.
SIGMA/A0157 - lyophilized powder, 1,000-3,000 units/mg protein
Synonym: L-Ascorbate:oxygen oxidoreductase; Ascorbase
CAS Number: 9029-44-1
EC Number: 232-852-6
MDL Number: MFCD00130560
Product Type: Chemical
Enzymatic Assay Enzymatic assay to measure the decrease in absorbance at 245 nm through exposure of L-ascorbic acid to Ascorbate oxidase and Oxygen. Stochiometry 2 L-ascorbic acid + oxygen + Ascorbate oxidase → 2 L-dehydroascorbic acid + 2 H2O
Enzymatic Assay Enzymatic assay to measure the decrease in absorbance at 245nm through exposure of L-ascorbic acid to Ascorbate oxidase and Oxygen. Stoichiometry: 2 L-ascorbic acid +oxygen + Ascorbate oxidase → 2 L-dehydroascorbic acid + 2 H2O
| biological source | plant (Cucurbita spp.) |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 1,000-3,000 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Ascorbate Oxidase from Cucurbita sp. has been used: • as a supplement in the culture medium for differentiation into osteoblasts • to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay • in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations |
| Application: | Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis . |
| Biochem/physiol Actions: | Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development. |
| Biochem/physiol Actions: | Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa. |
| General description: | Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria. |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Packaging: | 100, 250, 1000 units in poly bottle |
| Physical form: | Lyophilized powder containing buffers and sucrose as stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1,000-3,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.10.3.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |