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Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

SIGMA/A0162 - affinity isolated antibody, buffered aqueous solution

Synonym: Goat Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−AP

MDL Number: MFCD00162696
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A0162-.25ML 0.25 mL
$144.00
1/EA
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45-A0162-.5ML 0.5 mL
$219.00
1/EA
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45-A0162-1ML 1 mL
$397.00
1/EA
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antibody form affinity isolated antibody
antibody product type secondary antibodies
biological source goat
clone polyclonal
conjugate alkaline phosphatase conjugate
form buffered aqueous solution
Quality Level 200 
shipped in wet ice
species reactivity mouse
storage temp. 2-8°C
target post-translational modification unmodified
technique(s) direct ELISA: 1:3,000 using IgG, IgA, IgM
  western blot: 1:50 dilution
Application: Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.
Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352203

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