Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
SIGMA/A0168 - affinity isolated antibody
Synonym: Goat Anti-Mouse IgG (Fc specific)–HRP
MDL Number: MFCD00162629
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human appendix tissue sections stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:100 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A0168).
Western Blotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using 1:80,000 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A0168) and a chemiluminescent substrate. Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative control: without first antibody
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | |
| western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well |
| Application: | Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in • western blot • enzyme linked immunosorbent assay (ELISA) and • indirect immunoperoxidase assay (IIPA) |
| Application: | Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations. |
| Immunogen: | purified mouse IgG Fc fragment |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07,GHS07 |
| Signal word | Warning-Warning |
| Hazard statements | H317 - H317 |
| Precautionary statements | P261 - P272 - P280 - P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |