Anti-Human IgG (Fc specific)−Peroxidase antibody produced in goat
SIGMA/A0170 - affinity isolated antibody
Synonym: Anti Human IgG Antibody - Anti-Human IgG (Fc specific)-Peroxidase antibody produced in goat; Anti Human Igg; Anti Human Igg Hrp; Anti-Human Igg
MDL Number: MFCD00162439
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of tonsil stained with 1:200 Anti-Human IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A0170).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:60,000 |
| dot blot: 1:100,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (12 papers) Western Blotting (3 papers) |
| Application: | Goat anti-human IgG conjugated to peroxidase is suitable for use in ELISA (1:60,000), Dot Blot (1:100,000), immunohistochemistry (1:150) and affinity chromatography applications. |
| Application: | Peroxidase-conjugated goat anti-human IgG (Fc′ specific) antibody was used to detect the level of IgG activity in human plasma samples after treatment with rifin proteins by ELISA (1:50000). |
| Biochem/physiol Actions: | Antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Glycosylation is critical for IgG function. IgG subclasses have therapeutic potential against bacterial and viral infections. Gene translocation of the immunoglobulin heavy chain is associated in pathogenesis of multiple myeloma. |
| Biochem/physiol Actions: | Specificity of the Anti-Human IgG (Fc specific)- Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Cross-reactivity of the antibody-conjugate is determined by ELISA. The conjugate shows no reactivity with mouse or rat IgG and and yields reduced background with mouse or rat samples. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. The gene encoding IgG gene cluster is mapped to human chromosome 14. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. The antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and is purified to remove unconjugated material. Specificity and cross-reactivity of the Anti-Human IgG (Fc specific)-Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Furthermore, the conjugate shows no reactivity with mouse or rat IgG and yields reduced background with mouse or rat samples. |
| Immunogen: | Fc segment of human IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07,GHS07 |
| Signal word | Warning-Warning |
| Hazard statements | H317 - H317 |
| Precautionary statements | P261 - P272 - P280 - P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |