Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse
SIGMA/A0185 - clone BN-34, purified immunoglobulin
Synonym: Monoclonal Anti-Biotin
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:250 Anti-Human IgG (Fc specific)-Biotin antibody, Mouse monoclonal, Clone: HP-6017 (Cat. No. B3773), followed by 1:300 Monoclonal Anti-Biotin-Peroxidase antibody produced in Mouse, Clone: BN-34 (Cat. No. A0185).
ELISA 1:1,000 Anti-Human IgG (Fc specific)-Biotin antibody, Mouse monoclonal, Clone: HP-6017 (Cat. No. B3773), followed by serially diluted Monoclonal Anti-Biotin-Peroxidase antibody produced in Mouse, Clone: BN-34 (Cat. No. A0185), was probed to detect Human IgG by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BN-34, monoclonal |
| conjugate | peroxidase conjugate |
| isotype | IgG1 |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| flow cytometry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300 | |
| indirect ELISA: 1:30,000 | |
| western blot (chemiluminescent): 1:80,000 |
| Application: | May be substituted for avidin to decrease non-specific binding. |
| Application: | Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used: • in immunohistochemistry • in western blot • as secondary antibody to detect the presence of aptamer on the membrane |
| Application: | The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy. In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding. |
| Biochem/physiol Actions: | Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label. Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH). |
| General description: | The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates. |
| Immunogen: | Biotin-KLH conjugate |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |