Anti-l/s-Afadin antibody produced in rabbit
SIGMA/A0224 - affinity isolated antibody
Synonym: Anti-AF6; Anti-MLL-AF6; Anti-MLLT4; Anti-l-afadin
MDL Number: MFCD03455383
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluorescence. HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Anti-l-Afadin antibody produced in Rabbit (Cat. No. A0349) (A). The antibody developed using 1:50 Goat Anti-Rabbit IgG (whole molecule)-FITC conjugate antibody, and 1:500 Anti-l/s-Afadin antibody produced in Rabbit (Cat. No. A0224) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC Conjugate antibody. Results Two Anti-l/s-Afadin antibodies, A0349 (A) and A0224 (B) target different regions of l/s-Afadin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:250 Anti-l/s-Afadin antibody produced in Rabbit (Cat. No. A0224) followed by 1:500 Anti-Rabbit IgG-FITC antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| mol wt | antigen ~200 kDa (and 190 kDa) |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human, canine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections. | |
| indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells | |
| indirect immunofluorescence: 1:500 using frozen sections of mouse liver | |
| microarray: suitable | |
| western blot: 1:1000 using extract of rat brain. |
| Application: | Anti-l/s-Afadin antibody produced in rabbit has been used in : • immunocytochemistry • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin. |
| Biochem/physiol Actions: | s-Afadin binds to several cytoplasmic proteins such as the small guanosine triphosphatase (GTPase) Ras, the tight junction protein ZO-1 2 and the vinculin binding protein ponsin/ sorbin and SH3 domain containing 1 (SH3P12). In addition, l-afadin also binds through its PDZ several integral membrane components such as the Ca2+ -independent homophilic immunoglobulin-like nectin, the junctional adhesion molecule (JAM)4 and a subset of the EphB receptor protein-tyrosine kinases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins. |
| General description: | S-Afadin is a protein which lacks both the third proline-rich and the F-actin binding regions and is homologous to the human AF-6 (ALL-1 fusion partner from chromosome 6) gene product. Anti-l/s-Afadin is developed in rabbit using a synthetic peptide of rat afadin. |
| Immunogen: | synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |