Anti-Human IgG (Fab specific)−Peroxidase antibody produced in goat
SIGMA/A0293 - affinity isolated antibody
Synonym: Anti Human Fab Antibody; Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat
MDL Number: MFCD00162434
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with 1:200 Anti-Human IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A0293).
ELISA 10 μg/mL Anti-Human IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A0293), followed by 1:10,000 Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4187) was probed to detect Human IgG (Fab specific) by indirect ELISA. Reactivity is observed with Human IgG, Human Fab, Human IgA, Human IgM and Human K+L but not with Human Fc, Mouse IgG and Rat IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| dot blot: 1:100,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 | |
| western blot (chemiluminescent): 1:100,000 |
| Application: | Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in: • western blot • direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments. • immunohistochemistry • dot blot • immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma). |
| Application: | Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,. Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material. |
| Immunogen: | Fab fragment of human IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |