Anti-α1-Antitrypsin antibody produced in rabbit
SIGMA/A0409 - IgG fraction of antiserum
Synonym: Alpha 1 Antitrypsin Antibody; Alpha 1 Antitrypsin Antibody - Anti-α1-Antitrypsin antibody produced in rabbit; anti-A1AT
MDL Number: MFCD00162392
Product Type: Chemical
Western Blotting JOURNAL CITATION: Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules. By: Chapman, D. C., Stocki, P., et al. in PLoS One, 2015. PubMed ID: 26691022 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0145458), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
ELISA Anti-α1-Antitrypsin antibody produced in Rabbit (Cat. No. A0409), followed by 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect α1-Antitrypsin by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| mol wt | antigen ~50 kDa |
| packaging | vial of 2 mL lyophilized antiserum |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoelectrophoresis: suitable |
| indirect ELISA: 1:30,000-1:60,000 | |
| UniProt accession no. | P01009  |
| Application: | Anti-α1-antitrypsin antibody (diluted 1: 1000) can be used in ELISA. It can also be used for probing immunoblots to identify A1AT. Additionally, it can be used in Ouchterlony double diffusion. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Rabbit anti-α1-antitrypsin antibody can also be used in immunoelectrophoretic techniques. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-1-antitrypsin belongs to the serpin family and is a strong serine protease inhibitor. Its pivotal role is to protect lower respiratory tract against proteolytic destruction by human leukocyte elastase. Mutation in AAT gene will reduce the serum concentration of α-1-antitrypsin and hence increase the risk of emphysema. Anti-α1-antitrypsin antibody (diluted 1:9000) can be used as a capture antibody for determination of transgene expressions levels. It can also be used in immunoelectrophoresis. Rabbit anti-α1-antitrypsin antibody reacts specifically with α1-antitrypsin of human. |
| Immunogen: | Purified human α-1-antitrypsin |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |